About Us

Karen G. Carrasquillo, OD, PhD, FAAO
Director of Clinical Care

Karen G. Carrasquillo
OD, PhD, FAAO
Director - Clinical Care

Dr. Carrasquillo received her Doctor of Philosophy in Chemistry in 2001 from the University of Puerto Rico after graduating Magna Cum Laude with a Bachelors in Chemistry. The following year she completed a Postdoctoral Research Fellowship in Ophthalmology at  Massachusetts Eye and Ear. She continued her education at the New England College of Optometry where she received her Doctor of Optometry, followed by a Residency specializing in Cornea and Contact Lens at the New England College of Optometry. 

Dr. Carrasquillo is an adjunct clinical professor at the New England College of Optometry and at the School of Optometry, MCPHS University; an advisory member of the Gas Permeable Lens Institute; and Fellow of the American Academy of Optometry. She has also authored various publications in her field. 

Dr. Carrasquillo joined the staff at BostonSight in 2006. In 2014, Dr. Carrasquillo was promoted to Director of Clinical Care. In this role, Dr. Carrasquillo is responsible for BostonSight PROSE treatment delivery; and she works in conjunction with the medical director to define the BostonSight treatment model and clinical protocol.

“What I enjoy the most from my work at BostonSight is the satisfaction that comes from helping patients, restoring their vision and witnessing their regained sense of hope in life. Every day is literally a new day."

Curriculum Vitae

EDUCATION:

Fellow of the American Academy of Optometry, 2009.

Resident, Cornea and Contact Lens, New England College of Optometry, New England Eye Institute, Boston MA July 2005-July 2006. Affiliated Sites/Rotations: Boston Foundation for Sight, Cornea Consultants of Boston, Boston Medical Center, TLC The Laser Centers, Dimock Community Health Center.

Doctor of Optometry, New England College of Optometry, Boston, MA 2005. Massachusetts License #4552

Postdoctoral Research Fellow in Ophthalmology, Retina Service, Angiogenesis Laboratory, Harvard Medical School/Massachusetts Eye and Ear Infirmary, Boston MA 2001-2002. Principal Advisor: Dr. Anthony P. Adamis, Advisor: Dr. Joan W. Miller

Doctor of Philosophy, Chemistry, University of Puerto Rico, Río Piedras Campus, San Juan, PR 2001. Advisor: Dr. Kai Griebenow

Bachelor of Science, Magna Cum Laude, Chemistry, University of Puerto Rico, Río Piedras Campus, San Juan, PR 1996 .

WORKING EXPERIENCE:

Director of Clinical Care, BostonSight, Needham, MA.
January 2014 - present

Clinical Associate, BostonSight, Needham, MA.
August 2006- January 2014

• Responsible for the fitting and management of BostonSight® PROSE treatment in patients with corneal dystrophies and ocular surface diseases.
• Design and Conduct Clinical Research
• Preparation of Disclosures and Patent Applications
• Train Ophthalmology and Optometry clinicians in the fitting and management of PROSE

Senior Scientist, Eyetech Pharmaceuticals, Woburn, MA, 2002 – 2003

• Pioneered work on sustained polymer delivery devices for the antiangiogenic RNA aptamer (pegaptanib sodium) and protein antibodies for the treatment of neovascular (wet) age-related macular degeneration (AMD).
• Project leader in the development of transscleral drug delivery devices.
• Developed and conducted in vitro and ex vivo bioassays to determine bioactivity and bioavailability of the pegaptanib sodium (Macugen®). 
• Participated in the preparation of patent applications.
• Team member, Drug Delivery Due Diligence Package to establish the transition/collaboration with Pfizer Pharmaceuticals for the marketing of Macugen®.

ACADEMIC/TEACHING EXPERIENCE:
 
Adjunct Clinical Faculty, New England College of Optometry (Residency Program Affiliate), BostonSight, Needham, MA. July 2015 – present

• Clinic Director for Fourth year students clinical externship rotation
• Train and supervise optometry students in the management of PROSE treatment and further develop their clinical skills.

PROSE Treatment/Fellowship Course Clinical Instructor and Coordinator, BostonSight, Needham, MA  2008 – present
• Coordinate 9 week PROSE Fellowship Program for Optometrists at PROSE Partner Clinics
• PROSE treatment management and prosthetic device design, fitting and customization through use of CAD/CAM design software

Adjunct Clinical Faculty, New England College of Optometry (Residency Program Affiliate), Boston Foundation for Sight, Needham, MA. August 2006 – present
• Cornea and Contact Lens Residency Instructor
• Train and supervise Optometry Residents in fitting and management of PROSE devices and PROSE treatment

Clinical Preceptor/Instructor, New England College of Optometry, Boston, MA. January 2006 – 2008
• Contact Lens Laboratory instructor.
• Teach and guide optometry students, theory and clinical methods in contact lenses and contact lens fittings.

Instructor, Kaplan Educational Centers, San Juan, PR November 1997 – December 2001
• Teach General and Organic Chemistry test preparation courses for standardized tests such as MCAT, PCAT, and DAT. 
• Teaching appointments at the Kaplan Center, University of Puerto Rico-Mayagüez Campus, Catholic University of Puerto Rico, and University of Sacred Heart. 

Teaching Assistant, University of Puerto Rico, Río Piedras Campus, San Juan, PR August 1996 – May 1997
• General Chemistry Laboratory instructor.
• Teach undergraduate students theory and methods of chemistry experiments.

ACADEMIC HONORS AND AWARDS:
 
• American Optometric Foundation Travel Grant, American Academy of Optometry Annual Meeting, December 2005

• NIH Travel Grant, The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting, May 2002

• Beta Sigma Kappa International Optometric Honor Society 2004-2005

• Iaccoca Foundation Postdoctoral Research Fellowship 2001 - 2002

• GAANN Predoctoral Research Fellowship 1999 – 2001 

• NIH-SUBE Predoctoral Research Fellowship 1997 - 1998

• NIH-MARC Undergraduate Research Fellowship 1994 – 1996

RESEARCH FELLOWSHIPS:
 
Postdoctoral Research Fellowship, Harvard Medical School/MEEI, Ophthalmology Department 2001-2002
• Developed controlled delivery devices for the transscleral delivery of antiangiogenic molecules i.e., Macugen®. 
• Developed controlled delivery devices for protein antibodies for prevention of vascular proliferation, i.e., anti-VEGF, anti-ICAM-1.
• Conducted in vivo and in vitro assays for the determination of retinal-blood barrier breakdown and its prevention after transscleral delivery of antiangiogenic molecules.
• Co-authored a patent application: Carrasquillo, K.G. and Adamis A.P. (2004) “Drug Delivery Systems and Use Thereof”. Filed 2004, patent # 20050175708. Licensed to OSI Pharmaceuticals, February 2006.

Predoctoral Research Fellowship, University of Puerto Rico, Department of Chemistry, Río Piedras Campus, San Juan, PR. June 1994 – May 1996
• Contributed to the mutation of a P2-purinoceptor (P2U)
• Conducted experiments involving molecular biology techniques such as Polymerase Chain Reaction (PCR), synthesis of DNA primers and cloning. 

Summer Research Fellowship, National Institutes of Health, NIDDK, Bethesda, MD
• Crystallized the protein 3-Isopropylmalate dehydrogenase (IMDH) for its structure determination by X-ray Crystallography. 

Summer Research Fellowship, National Institutes of Standard and Technology, Gaithesburg, MD 1994.

PEER REVIEWED PUBLICATIONS (17):

•DeLoss, K.D., Le H-G, Gire, A., Chiu, G.B, Jacobs, D.S, and Carrasquillo, K.G., (2015) PROSE Treatment of Ocular Chronic Graft-Versus-Host Disease as a Clinical Network Expands. Eye and Contact Lens., Epub ahead of print, October 2015.

•Hussoin T, Le HG, Carrasquillo KG, Johns L, Rosenthal P, Jacobs DS. The Effect of Optic Asphericity on Visual Rehabilitation of Corneal Ectasia with a Prosthetic Device. Eye Contact Lens. 2012 Sep;38(5):300-5.

• Sleeper, A., Jacobs, D.S., and Carrasquillo, K.G. (2011) Management of vascularized limbal keratitis with prosthetic replacement of the ocular surface system. Eye Contact Lens. 2012 Mar;38(2):137-40.

• Languis, M., and Carrasquillo, K.G. (2010) Piggy-back cosmetic contact lens as an occlusion therapy in a patient with familial dysautonomia. Eye & Contact Lens: Science & Clinical Practice. 36(6) 367-370.

• Jacobs, D.S., Lim, M., Carrasquillo, K.G., and Rosenthal, P. (2009) Bevacizumab for corneal neovascularization. Ophthalmol. 116(3) 592-593. 

• Jacobs, D.S., Carrasquillo, K.G., and Rosenthal, P (2009) Boston Scleral Lens Prosthesis as a novel corneal drug delivery system. Semin. Ophthalmol., 24(3) 149-155.

• Carrasquillo, K.G., Rand, J., and Talamo, J.H. (2007) Intacs for keratoconus and post-LASIK ectasia: Mechanical versus Femtosecond laser assisted channel creation. Cornea, 26(8), 956-62.

• Ishida, S., Usui, T., Yamashiro, K., Kaji, Y., Ahmed, E., Carrasquillo, K.G., Hida, T., Oguchi, Y., and Adamis, A.P. (2003) VEGF 164 is proinflammatory in the diabetic retina. Invest. Ophthalmol. Vis. Sci., 44(5), 2155-2162.

• Carrasquillo, K.G., Ricker, J.A., Rigas, I.K., Miller, J.W., Gragoudas, E.S, and Adamis, A.P. (2003) Controlled delivery of anti-VEGF aptamer EYE001 with poly(lactic-co¬-glycolic)acid microspheres. Invest. Ophthalmol. Vis. Sci., 44(1), 290-299.

• Costantino, H.R., Firouzabadian, L., Wu, C., Carrasquillo, K.G., Griebenow, K., Zale, S.E., and Tracy, M. (2002) Protein Spray-Freeze Drying. 2. Effect of formulation variables on particle size and stability. J. Pharm. Sci., 91, 388-95.

• Carrasquillo, K.G., Stanley, A.M., Aponte-Carro, J.C., De Jesús, P., Costantino, H.R., Bosques, C.J., and Griebenow, K. (2001) Non-aqueous encapsulation of excipient-stabilized spray-freeze dried BSA into poly(lactide-co¬-glycolide) microspheres results in release of native protein. J. Control. Rel., 76, 199-208.

• Castellanos, I.J., Carrasquillo, K.G., De Jesús, J., Alvarez, M., and Griebenow, K. (2001) Encapsulation of bovine serum albumin in poly(lactide-co-glycolide) microspheres by the solid-in-oil-in-water technique. J. Pharm. Pharmacol., 53, 167-178.

• Costantino, H.R., Firouzabadian, L., Hogeland K., Wu, C., Beganski, C., Carrasquillo, K.G., Córdova, M., Griebenow, K., Zale, S.E, and Tracy, M. (2001) Protein Spray-Freeze Drying. Effect of Atomization Conditions on Particle Size and Stability. Pharm. Res., 17, 1374-1383.

• Carrasquillo K.G., Sanchez C., and Griebenow K. (2000) Relationship between conformational stability and lyophilization-induced structural changes in chymotrypsin. Biotechnol. Appl. Biochem. 31, 41-53.

• Carrasquillo, K.G., Costantino, H.R., Cordero, R.A., Hsu, C.C., and Griebenow, K. (1999) On the structural preservation of recombinant human growth hormone in a dried film of a synthetic biodegradable polymer. J. Pharm. Sci. 88, 166-173. 

• Carrasquillo, K.G., Cordero, R.A., Ho, S., Franquiz, J.M., and Griebenow, K. (1998) Structure-guided encapsulation of bovine serum albumin in ploy(DL-lactic-co-glycolic)acid. Pharm. Pharmacol. Commun. 4, 563-571.

• Costantino, H.R., Carrasquillo, K.G., Cordero, R.A., Mumenthaler, M., Hsu, C.C., and Griebenow, K. (1998) The effect of excipients on the structure and stability of lyophilized recombinant human growth hormone (rhGH). J. Pharm. Sci. 87, 1412-1420.

NON PEER REVIEWED PUBLICATIONS:

• Carrasquillo, K.G., and Gregory DeNaeyer, (2015) Scleral lenses for severely diseased eyes. Feature article in October 2015 issue of Contact Lens Spectrum.

• Carrasquillo, K.G., Kwok, A., and Patel, C. (2014) Severe forms of ocular surface disease. Feature article in July 2014 issue of Contact Lens Spectrum.

• Carrasquillo, K.G., and Barnett, M. (2014) Scleral lens applications in unique patient populations. Feature article in February 2014 issue of Contact Lens Spectrum.

• Carrasquillo, K.G. (2001) Structure-guided encapsulation of proteins into biocompatible polymers. Doctoral Thesis. ProQuest Information and Learning. Catalog # 3032982.

• Griebenow K., Santos A.M., and Carrasquillo KG. (1999) Secondary structure of proteins in the amorphous dehydrated state probed by FTIR spectroscopy. Dehydration-induced structural changes and their prevention. Internet J. Vib. Spec. 3 (1), [www.ijvs.com]

• Griebenow K., Castellanos I., and Carrasquillo KG. (1999) Application of FTIR spectroscopy to probe and improve protein structure in sustained release devices. Int. J. Vibr. Spec., [www.ijvs.com] 3, 5, 2.

PATENTS :

• Carrasquillo, K.G. and Adamis A.P. (2004) Drug Delivery Systems and Use Thereof. Patent filed 2004, patent # 20050175708. Licensed to OSI Pharmaceuticals, February 2006.

SEMINAR/POSTER PRESENTATIONS:

• Karen G. Carrasquillo (2015) Long-term restoration of ocular surface function with scleral lenses. Lecture. European association for Vision and Eye Research Conference, Nice, France. October 7-10, 2015.

• Karen G. Carrasquillo (2015) Corneal disease and scleral lenses. Lecture. Massachusetts Society of Optometrists Spring CE Conference, Marlborough, MA. March 15, 2015.

• Karen G. Carrasquillo (2014) Use of PROSE prosthetic device as treatment option for paralytic lid ptosis post open globe injury and trauma in a pediatric patient. Poster presentation. Annual Meeting American Academy of Optometry, Denver, CO. November 12-15, 2014.

• Karen G. Carrasquillo (2013) Ellerbrock Presents Grand Rounds, Part III, When lightning strikes twice. Annual Meeting American Academy of Optometry, Seattle, WA. October 23-26, 2013.

• Karen G.  Carrasquillo (2012) Ellerbrock Presents Grand Rounds, Part III, Persistent Epithelial Defect and Dense Central Corneal Scarring Management with PROSE. Annual Meeting American Academy of Optometry, Phoenix, AZ. October 24-27, 2012.

• Karen Deloss, Karen Carrasquillo, Hong-Gam Le, Deborah S. Jacobs. (2012, May). PROSE Treatment for Ocular Chronic Graft-Versus-Host Disease as a Clinical Network Expands. Presented at IOSS Annual Meeting, Ft. Lauderdale, FL.

• Deborah S. Jacobs, Hong-Gam T. Le, Karen G. Carrasquillo (2012) Impact of treatment of ocular chronic GVHD with a scleral lens prosthetic device as a national network of clinical centers expands. Bone-marrow Tandem Meetings, San Diego, CA. February 1-5, 2012

• Karen G. Carrasquillo. Inspiring Minds: Meet women in science. Invited speaker. Museum of Science, Boston MA. April 29, 2011.

• Karen G. Carrasquillo, Ana Sleeper, and Deborah S. Jacobs (2010) Management of Vascularized Limbal Keratitis with the Boston Ocular Surface Prosthesis. American Academy of Optometry Annual Meeting. November 17-10, 2010.

• Karen G. Carrasquillo, Albert Swiston, Erik Williamson (2007) Surface Modification of the Boston Scleral Lens. IDEAS Competition: Team BostonSight, Massachusetts Institute of Technology, Cambridge, MA.

• Karen G. Carrasquillo (2006) Soft Multifocal Contact Lens Fitting. Bausch and Lomb sponsored Advanced Contact Lens Workshop, New England College of Optometry, Boston, MA. (Oral Presentation)

• Karen G. Carrasquillo (2006) Intacs for keratoconus and post-Lasik ectasia. The John E. Asarkof Residency Conference. New England College of Optometry, Bedford VA Medical Center, Bedford, MA. May 18th, 2006. (Oral Presentation)

• Karen G. Carrasquillo (2005) Gas permeable contact lens, solutions, and designs. Bausch and Lomb sponsored Advanced Contact Lens Workshop, New England College of Optometry, Boston, MA. (Oral Presentation)

• Karen G. Carrasquillo, Ronald K. Watanabe (2005) Custom Bi-Symmetrical lens fit provides viable alternative for irregular corneas in advanced keratoconus. American Academy of Optometry Annual Meeting. December 8-11, San Diego, CA, 2005.

• Karen G. Carrasquillo, Subhransu Ray, Ioannis Rigas, Perry Calias, Joan W. Miller, Evangelous S. Gragoudas, and Anthony P. Adamis (2003) Transscleral delivery of an anti-VEGF aptamer in a rabbit model. The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting. May 4-9, Fort Lauderdale, Fl, 2003.
    
• Subhransu Ray, Karen G. Carrasquillo, Ioannis Rigas, Joan W. Miller, Evangelous S. Gragoudas, and Anthony P. Adamis (2003) Encapsulation of ah-ICAM1 antibody in PLA-PEG microspheres: In vitro and in vivo characterization. The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting. May 4-9, Fort Lauderdale, Fl, 2003.
                    
• Karen G. Carrasquillo, Joseph A. Ricker, Ioannis K. Rigas, and Anthony P. Adamis (2002) Controlled delivery of anti-VEGF aptamer EYE001 with poly(lactic-co¬-glycolic)acid microspheres. The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting. May 5-10, Fort Lauderdale, Fl, 2002.
                                                               
• Karen G. Carrasquillo (2002) Biodegradable microspheres for sustained drug delivery: Applications for ocular drug delivery. New England College of Optometry, Boston, MA. (Oral presentation/Invited Speaker).

• Karen G. Carrasquillo (2000) Stabilization of proteins encapsulated in PLGA for their sustained-release. Schepens Eye Research Institute, Harvard Medical School, Boston, MA. (Oral presentation).

• Karen G. Carrasquillo, Adriana L. Alejandro, and Kai Griebenow (1999) Non-aqueous microencapsulation of bovine serum albumin (BSA) in PLGA, 5th US-Japan Symposium on Drug Delivery Systems, Maui, Hawaii, Dec. 12-17, 1999. (Poster selected for oral presentation).

• Karen G. Carrasquillo, Cheryll Sanchez, and Kai Griebenow (1999) Structure-guided encapsulation of proteins in biocompatible polymers. The Protein Society, Thirteenth Symposium, July 24-28, 1999, Boston, Massachusetts.

• Karen G. Carrasquillo, Cheryll Sanchez, Rocio A Cordero, and Kai Griebenow (1999) Structure-guided encapsulation of proteins in biocompatible polymers. ASBMB meeting, San Francisco (May 16-20, 1999)

• Karen G. Carrasquillo, Rocio A. Cordero, Henry R. Costantino, Marco Mummenthaler, Chung C. Hsu and Kai Griebenow (1998) Improved solid-state stability of recombinant human growth hormone (rhGH), Meeting of the Protein Society, July 25-29, 1998, San Diego, Ca.

• Karen G. Carrasquillo, Rocío A. Cordero, Edward McBurney, Juan M. Franquiz, and Kai Griebenow (1998) Solid-state structure and stability of bovine serum albumin (BSA). 10th PR-EPSCoR Annual Meeting, Rio Grande, PR, May 22-24, 1998.

• Karen G. Carrasquillo, H.R. Costantino, C.C. Hsu, and Kai Griebenow (1998) Preservation of the Native Secondary Structure of Recombinant Human Growth Hormone (rhGH) in Organic Solvents. 33rd ACS Junior Technical Meeting, March 7,1998, Interamerican University Puerto Rico, Bayamón Campus.

• Karen G. Carrasquillo, Rocio A. Cordero, Henry R. Costantino, Marco Mummenthaler, Chung C. Hsu and Kai Griebenow (1997) Investigations on the Solid-State Stability of Lyophilized Recombinant Human Growth Hormone.

RESEARCH INTERESTS:
 
• Ophthalmic Drug Delivery
• Use of polymer devices, including contact lenses for the delivery of anterior segment drugs and uses thereof.
• Dry Eye Syndrome.
• Clinical Research.

SERVICE – PROFESSIONAL:
 
Reviewer for Professional Journals 
• Journal of Pharmaceutical Science, 2001
Professional Affiliations 
• American Optometric Student Association 2002- present
• American Chemical Society 1994 – 2000

SERVICE -- UNIVERSITY:

• UPR Department of Chemistry, Graduate Program Student Representative 1997 - 1998
• UPR American Chemical Society (ACS) Student Affiliate Chapter, Vice-President 1995 – 1996. 
• UPR American Chemical Society (ACS) Student Affiliate Chapter, Treasurer 1994 – 1995
 

Senior Staff

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Gene Guselli, President and CEO
Gene Guselli, President and CEO

Gene P. Guselli
President and CEO

Melinda Kimball, Human Resources and Payroll Manager
Melinda Kimball, Human Resources and Payroll Manager

Melinda Kimball
Human Resources and Payroll Manager

Ilene Knopping, Director of the BostonSight® Network
Ilene Knopping, Director of the BostonSight® Network

Ilene Knopping
Director of the BostonSight® Network

Darlene Riordan, Clinical Manager
Darlene Riordan, Clinical Manager

Darlene Riordan
Clinical Operations Manager

Tara Stepanian, Manager of Annual Giving Progrmas
Tara Stepanian, Manager of Annual Giving Progrmas

Tara Stepanian
Manager of Annual Giving Programs

Olga Tomashevaskaya, Director of Engineering
Olga Tomashevaskaya, Director of Engineering

Olga Tomashevskaya
Director of Engineering

Sara Yost, Chief Financial Officer
Sara Yost, Chief Financial Officer

Sara Yost
Chief Financial Officer

Staff

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Colin Bateman
Colin Bateman

Lab Technician

Pedro Araujo
Pedro Araujo

Pedro Araujo
Lab Technician

Manoel Carvalho
Manoel Carvalho

Manoel Carvalho
Lab Manager

Artur Barbosa
Artur Barbosa

Artur Barbosa
Lab Technician

Lynn Carter
Lynn Carter

Lynn Carter
Senior Patient Claims Representative

Kerri Cruz
Kerri Cruz
Rita DeFlaminis
Rita DeFlaminis

Rita DeFlaminis
Returning Patient Affairs Coordinator

Elizabeth Gibson
Elizabeth Gibson

Elizabeth Gibson
Clinic Technician

Daniel Gosselin
Daniel Gosselin

Daniel Gosselin
Lab Technician

Sabrina Grankewicz
Sabrina Grankewicz

Sabrina Grankewicz

Durable Medical Equipment Coordinator

Yudit Guzman
Yudit Guzman

Yudit Guzman
Lead Ophthalmic Technician

Kim Levin
Kim Levin

Klimenty Levin
Software/Systems Engineer

Nancy Lemist
Nancy Lemist

Nancy Lemist
Clinical Support Manager

David Loomis
David Loomis

David Loomis
Financial Administrator

Isabell Ofgant
Isabell Ofgant

Isabell Ofgant
Patient Claims Representative

 

Sue Sharer
Sue Sharer

Sue Sharer

Software Quality Assurance Engineer

Loren Stead
Loren Stead

Loren Stead
Patient Insurance Coordinator

Heidi Wolfe
Heidi Wolfe

Heidi Wolfe
Clinic Technician